3STB
A complex of two editosome proteins and two nanobodies
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2010-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792, 0.9794, 0.9116 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.540, 74.540, 239.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.530 - 2.500 |
R-factor | 0.20069 |
Rwork | 0.199 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.328 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.530 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.125 | 0.361 |
<I/σ(I)> | 17.49 | 2.333 |
Completeness [%] | 97.1 | 77.3 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 10% 2-propanol, 22% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 10% 2-propanol, 22% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |