3SSK
Engineered high-affinity halide-binding protein derived from YFP: bromide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.91993 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.379, 62.361, 69.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.454 - 1.361 |
R-factor | 0.1316 |
Rwork | 0.129 |
R-free | 0.17150 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3sry |
RMSD bond length | 0.010 |
RMSD bond angle | 1.288 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.454 |
High resolution limit [Å] | 1.361 |
Number of reflections | 47146 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 17% PEG2000, 150 mM sodium acetate, 90 mM magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |