3SS3
Crystal structure of mouse Glutaminase C, ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.260, 138.810, 179.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.934 - 2.420 |
R-factor | 0.1981 |
Rwork | 0.195 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3czd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.550 |
High resolution limit [Å] | 2.420 | 2.420 |
Number of reflections | 89075 | |
<I/σ(I)> | 5.8 | 1.8 |
Completeness [%] | 94.2 | 89.5 |
Redundancy | 4.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 17% PEG3350, 0.2M NaCl, 0.1M Bis-TRIS, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |