3SRN
STRUCTURAL CHANGES THAT ACCOMPANY THE REDUCED CATALYTIC EFFICIENCY OF TWO SEMISYNTHETIC RIBONUCLEASE ANALOGS
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.700, 64.700, 64.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.700 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.078 * |
| Number of reflections | 9604 * |
| Completeness [%] | 84.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macro seeding * | 5.2 * | THE MOTHER LIQUOR WAS 80 PERCENT SATURATED AMMONIUM SULFATE, PH 5.2. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNase1-118 | 0.95 (mM) | |
| 2 | 1 | drop | RNase111-124 | 9.9 (mM) | |
| 3 | 1 | reservoir | 3.0 (M) | ||
| 4 | 1 | reservoir | ammonium sulfate | 28 (%sat) | |
| 5 | 1 | reservoir | ammonium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | 1,10-phenanthroline | 5 (mM) |
