3SQX

Structure of Mss116p (NTE and C-tail double deletion) bound to ssRNA and AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	93
Detector technology	CCD
Collection date	2009-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 2
Unit cell lengths	88.943, 127.043, 55.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.610 - 2.112
R-factor	0.21696
Rwork	0.215
R-free	0.26012
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I5X CHAIN A
RMSD bond length	0.011
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0109)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	34638
<I/σ(I)>	17.7	1.7
Completeness [%]	95.3	83.8
Redundancy	6.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M ammonium tartrate dibasic, pH 7.0, 10% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K