3SQW

Structure of Mss116p (NTE deletion) bound to ssRNA and AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	93
Detector technology	CCD
Collection date	2009-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 2
Unit cell lengths	88.159, 126.630, 56.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.530 - 1.909
R-factor	0.1933
Rwork	0.191
R-free	0.23729
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I5X CHAIN A
RMSD bond length	0.011
RMSD bond angle	1.292
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0109)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	48576
<I/σ(I)>	30.3	2.1
Completeness [%]	98.1	87.1
Redundancy	13.3	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	100 mM ammonium acetate, 20 mM MgCl2, 50 mM HEPES-Na pH 7.0, and 5% polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295K