3SQN
Putative Mga family transcriptional regulator from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.803, 101.759, 89.902 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 36.170 - 2.310 |
| R-factor | 0.2266 |
| Rwork | 0.224 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.651 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.200 | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 6.290 | 2.320 |
| Rmerge | 0.070 | 0.045 | 0.627 |
| Number of reflections | 48502 | ||
| <I/σ(I)> | 9.8 | 2.1 | |
| Completeness [%] | 97.5 | 96.1 | 81 |
| Redundancy | 5.1 | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 2.0 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
