3SQM
Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 125.139, 125.139, 233.745 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.961 - 2.703 |
R-factor | 0.163 |
Rwork | 0.159 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_761)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 58559 | |
<I/σ(I)> | 8.9 | 2.2 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 289 | 0.1M ammonium acetate 0.1M BIS-TRIS pH 6.8, 23.3% PEG 3350, 5.3mM N-ACETYL-D-GLUCOSAMINE, VAPOR DIFFUSION, SITTING DROP, temperature 289K |