3SPW
Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9334 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.460, 54.760, 121.860 |
Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
Resolution | 47.290 - 2.600 |
R-factor | 0.2302 |
Rwork | 0.228 |
R-free | 0.26960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zhw |
RMSD bond length | 0.002 |
RMSD bond angle | 0.596 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.290 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 30180 | |
<I/σ(I)> | 6.82 | 2.78 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 250 mM CaCl2, 100 mM MES, 16% PEG 20K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |