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3SPJ

Apo inward rectifier potassium channel Kir2.2 I223L mutant

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X29A
Synchrotron siteNSLS
BeamlineX29A
Temperature [K]100
Detector technologyCCD
Collection date2011-06-27
DetectorADSC QUANTUM 315
Spacegroup nameI 4
Unit cell lengths83.769, 83.769, 197.126
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution28.364 - 3.307
R-factor0.2365
Rwork0.235
R-free0.26680
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3sph
RMSD bond length0.008
RMSD bond angle0.877
Refinement softwarePHENIX ((phenix.refine: 1.7_650))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.400
High resolution limit [Å]3.3003.300
Rmerge0.0120.053
Number of reflections10028
<I/σ(I)>14.53.7
Completeness [%]99.099.8
Redundancy7.57.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP727710.62% PEG400, 0.5 M sodium chloride, 0.05 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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