3SPJ
Apo inward rectifier potassium channel Kir2.2 I223L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-27 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 4 |
Unit cell lengths | 83.769, 83.769, 197.126 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.364 - 3.307 |
R-factor | 0.2365 |
Rwork | 0.235 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sph |
RMSD bond length | 0.008 |
RMSD bond angle | 0.877 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.012 | 0.053 |
Number of reflections | 10028 | |
<I/σ(I)> | 14.5 | 3.7 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 7.5 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10.62% PEG400, 0.5 M sodium chloride, 0.05 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |