3SPI

Inward rectifier potassium channel Kir2.2 in complex with PIP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	I 4
Unit cell lengths	83.326, 83.326, 184.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.357 - 3.307
R-factor	0.2373
Rwork	0.235
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sph
RMSD bond length	0.012
RMSD bond angle	0.835
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.083	0.064
Number of reflections	9392
<I/σ(I)>	16.5	1.8
Completeness [%]	99.0	100
Redundancy	5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	10.62% PEG4000, 0.5 M potassium chloride, 0.05 M HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K