3SPC

Inward rectifier potassium channel Kir2.2 in complex with dioctanoylglycerol pyrophosphate (DGPP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	82.904, 82.904, 191.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.657 - 2.454
R-factor	0.1944
Rwork	0.193
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jyc
RMSD bond length	0.011
RMSD bond angle	0.882
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	2.600
High resolution limit [Å]	2.450	2.450
Number of reflections	23341
<I/σ(I)>	11.7	2.2
Completeness [%]	100.0	100
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP, VAPOR DIFFUSION	7		6.2% PEG400, 0.5 M potassium chloride, 0.1 M HEPES, pH 7.0, HANGING DROP, VAPOR DIFFUSION