3SPC
Inward rectifier potassium channel Kir2.2 in complex with dioctanoylglycerol pyrophosphate (DGPP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 82.904, 82.904, 191.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.657 - 2.454 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jyc |
RMSD bond length | 0.011 |
RMSD bond angle | 0.882 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.000 | 2.600 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 23341 | |
<I/σ(I)> | 11.7 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP, VAPOR DIFFUSION | 7 | 6.2% PEG400, 0.5 M potassium chloride, 0.1 M HEPES, pH 7.0, HANGING DROP, VAPOR DIFFUSION |