3SP3
Lysozyme in 20% sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.847, 76.847, 37.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.410 - 1.801 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rnx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.790 |
| Rmerge | 0.061 | 0.391 |
| Number of reflections | 11095 | |
| <I/σ(I)> | 33.9 | 2.09 |
| Completeness [%] | 92.9 | 33.1 |
| Redundancy | 6.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 277 | 40 mM Sodium Acetate, 150mM Sodium Chloride, 20% Sucrose, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
