3SOX

Structure of UHRF1 PHD finger in the free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 21 21 21
Unit cell lengths	53.746, 53.805, 128.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.013 - 2.650
R-factor	0.2504
Rwork	0.248
R-free	0.29430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3SOU CHAIN A
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.740
High resolution limit [Å]	2.650	5.700	2.650
Rmerge	0.064	0.029	0.845
Number of reflections	5681
<I/σ(I)>	18.9		3.1
Completeness [%]	99.5	98.6	99.1
Redundancy	7	6.4	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2 M ammonium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K