3SOW
Structure of UHRF1 PHD finger in complex with histone H3K4me3 1-9 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.622, 42.622, 183.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.778 - 1.950 |
R-factor | 0.2052 |
Rwork | 0.203 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3SOU CHAIN A |
RMSD bond length | 0.008 |
RMSD bond angle | 1.115 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.120 | 0.055 | 0.781 |
Number of reflections | 13122 | ||
<I/σ(I)> | 11.2 | 3.23 | |
Completeness [%] | 98.7 | 98.2 | 96.1 |
Redundancy | 13.3 | 14.3 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% w/v polyethylene glycol 8,000, 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |