3SOL
Crystal structure of the type 2 secretion system pilotin GspS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 73.350, 73.350, 70.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.260 - 1.900 |
R-factor | 0.1916 |
Rwork | 0.190 |
R-free | 0.22160 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.102 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.261 | 1.950 | |
High resolution limit [Å] | 1.900 | 8.480 | 1.900 |
Rmerge | 0.085 | 0.010 | 0.010 |
Number of reflections | 16858 | 192 | 1225 |
<I/σ(I)> | 24.39 | 94.57 | 2.47 |
Completeness [%] | 99.9 | 99.5 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M MG CHLORIDE, 0.1M TRIS-HCL, 30% PEG3350, pH 8.5, vapor diffusion, sitting drop, temperature 298K |