3SOH

Architecture of the Flagellar Rotor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	91.390, 91.390, 226.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.210 - 3.500
R-factor	0.253
Rwork	0.253
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2HP7 AND 1LKV
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.210	3.630
High resolution limit [Å]	3.500	3.500
Number of reflections	13308
<I/σ(I)>	20.5	4.1
Completeness [%]	91.9	84.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5		0.1 M MES, pH 6.5, 10% dioxane, 1.6 M ammonium sulfate, EVAPORATION