3SOB
The structure of the first YWTD beta propeller domain of LRP6 in complex with a FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979464 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.037, 83.363, 62.844 |
| Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
| Resolution | 29.071 - 1.900 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Number of reflections | 57170 | ||
| Completeness [%] | 91.9 | 97.8 | 57.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M NaCl, 0.1 M Tris pH 8, 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
