3SNS
Crystal structure of the C-terminal domain of Escherichia coli lipoprotein BamC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98058 |
Spacegroup name | H 3 |
Unit cell lengths | 78.850, 78.850, 52.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.820 - 1.500 |
R-factor | 0.16195 |
Rwork | 0.161 |
R-free | 0.18321 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yh5 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.596 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.820 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.075 | 0.378 |
Number of reflections | 19576 | |
<I/σ(I)> | 10.3 | 3.2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M sodium chloride, 0.1 M HEPES, pH 6.5, 1.6 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |