3SNS
Crystal structure of the C-terminal domain of Escherichia coli lipoprotein BamC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98058 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.850, 78.850, 52.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.820 - 1.500 |
| R-factor | 0.16195 |
| Rwork | 0.161 |
| R-free | 0.18321 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yh5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.820 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.075 | 0.378 |
| Number of reflections | 19576 | |
| <I/σ(I)> | 10.3 | 3.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M sodium chloride, 0.1 M HEPES, pH 6.5, 1.6 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
