3SNK
Crystal structure of a Response regulator CheY-like protein (mll6475) from MESORHIZOBIUM LOTI at 2.02 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.859, 64.859, 69.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.805 - 2.020 |
R-factor | 0.1988 |
Rwork | 0.198 |
R-free | 0.22210 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.805 | 2.090 | |
High resolution limit [Å] | 2.020 | 4.340 | 2.020 |
Rmerge | 0.075 | 0.056 | 0.969 |
Number of reflections | 11550 | 2186 | 2063 |
<I/σ(I)> | 18.05 | 39.2 | 2.7 |
Completeness [%] | 99.7 | 99.9 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.0% polyethylene glycol 400, 2.0M ammonium sulfate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |