3SM3
Crystal Structure of SAM-dependent methyltransferases Q8PUK2_METMA from Methanosarcina mazei. Northeast Structural Genomics Consortium Target MaR262.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97908 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 51.589, 51.589, 207.028 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.817 - 2.197 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.151 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.197 | 2.200 |
Rmerge | 0.107 | 0.723 |
Number of reflections | 54511 | |
<I/σ(I)> | 20.1 | 2.38 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 7.5 | 277 | 27% PEG 400, 0.2 M calcium chloride, 5% glycerol, 0.1 M HEPES, pH 7.5 , Microbatch crystallization under oil , temperature 277K |