3SM0
tRNA-Guanine Transglycosylase in complex with lin-Benzohypoxanthine Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.950, 64.926, 71.229 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.570 |
| R-free | 0.18230 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 54458 | |
| <I/σ(I)> | 16.2 | 2.74 |
| Completeness [%] | 93.8 | 83.9 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 100MM TRIS HCL, 1MM DTT, 10% DMSO, 5% PEG 8000 , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
