3SLC
Crystal structure of apo form of acetate kinase (AckA) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 283.159, 62.168, 91.695 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iir |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.138 | 0.558 |
| Number of reflections | 42614 | |
| <I/σ(I)> | 2.06 | |
| Completeness [%] | 96.9 | 84.5 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | UNDER-OIL MICROBATCH | 7.5 | 298 | 0.1M HEPES, 30%(w/v) PEG 4000, 0.2M Calcium Chloride, pH 7.5, UNDER-OIL MICROBATCH, temperature 298K |
