3SKW
Crystal structure of the 2'- Deoxyguanosine riboswitch bound to 2'- Deoxyguanosine, cesium soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.376, 35.414, 119.269 |
Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
Resolution | 19.646 - 2.950 |
R-factor | 0.2242 |
Rwork | 0.217 |
R-free | 0.28730 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.045 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.108 | 0.335 |
Number of reflections | 8847 | |
<I/σ(I)> | 19.6 | 4.7 |
Completeness [%] | 95.7 | 98.4 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M Na-HEPES, pH 7.5, ~40 % (v/v) pentaerythritol propoxylate, 0.2 M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |