3SKS
Crystal structure of a putative oligoendopeptidase F from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.984, 84.551, 116.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| R-factor | 0.18353 |
| Rwork | 0.181 |
| R-free | 0.23381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ahn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.085 | 0.700 |
| Number of reflections | 39935 | |
| <I/σ(I)> | 23.1 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20%PEG 3350,0.2M K dihydrogen Phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
