3SKR
Crystal structure of the 2'- Deoxyguanosine riboswitch bound to 2'- Deoxyguanosine, cobalt Hexammine soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.45 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.530, 35.018, 110.761 |
Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.24157 |
Rwork | 0.236 |
R-free | 0.29872 |
Structure solution method | Phaser |
RMSD bond length | 0.006 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.497 | |
Number of reflections | 7041 | |
<I/σ(I)> | 3.9 | |
Completeness [%] | 96.8 | 98.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M Na-HEPES, pH 7.5, ~40 % (v/v) pentaerythritol propoxylate, 0.2 M KCl and 2.0 mM spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |