3SKQ
Mdm38 is a 14-3-3-like receptor and associates with the protein synthesis machinery at the inner mitochondrial membrane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.527, 52.033, 47.232 |
Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.144 | 0.762 |
Number of reflections | 16561 | |
<I/σ(I)> | 15.8 | 3.11 |
Completeness [%] | 97.8 | 97.6 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.25M KI and 21% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |