3SKB
Structural characterization of a GII.4 2004 norovirus variant (TCH05)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-14 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 243.910, 339.120, 125.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.566 - 3.220 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sld |
RMSD bond length | 0.011 |
RMSD bond angle | 1.318 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.566 | 3.390 |
High resolution limit [Å] | 3.220 | 3.220 |
Rmerge | 0.171 | 0.361 |
Number of reflections | 79184 | |
<I/σ(I)> | 7.8 | 3.9 |
Completeness [%] | 94.2 | 93.7 |
Redundancy | 3.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 0.2M Sodium citrate, 0.1M HEPES and 20% Isoproponol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |