3SK5
Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.110, 60.666, 38.269 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 27.650 - 1.700 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.731 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.051 | 0.046 | 0.191 |
| Number of reflections | 15185 | ||
| <I/σ(I)> | 27.6 | ||
| Completeness [%] | 87.9 | 84.4 | 48.9 |
| Redundancy | 5.4 | 5.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
