3SJZ
The structure of aIF2gamma subunit delta 41-45 from archaeon Sulfolobus solfataricus complexed with GDP and GDPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 203.6 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.89430 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 94.750, 94.750, 165.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.414 - 2.800 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aho |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 21693 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 301 | 4.5% sodium malonate, 5.5mM GDP+GDPNP, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
