3SJS

Crystal structure of URE3-binding protein from Entamoeba histolytica, (D127A,N129A) mutant, native form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-16
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.541780
Spacegroup name	C 2 2 21
Unit cell lengths	46.250, 68.290, 130.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.290 - 1.900
R-factor	0.174
Rwork	0.172
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sia
RMSD bond length	0.016
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.290		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.055	0.036	0.431
Number of reflections	16665	217	1189
<I/σ(I)>	17.42	48.4	2.4
Completeness [%]	99.4	97.7	96.3
Redundancy	5.05		2.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	290	Internal tracking number 215760A3. Focus screen based on CryoFull G6. 1 uL 40% PEG600, 0.1 M citrate pH 4.56 + 1 uL EnhiA.01648.a.D18 PD00123 (VCID3088) at 9.5 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 290K