3SJP
Structural characterization of a GII.4 2004 norovirus variant (TCH05)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-17 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.001, 72.966, 55.610 |
| Unit cell angles | 90.00, 97.78, 90.00 |
Refinement procedure
| Resolution | 28.108 - 2.004 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2obs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 2.004 | 2.004 |
| Rmerge | 0.054 | 0.300 |
| Number of reflections | 23043 | |
| <I/σ(I)> | 19.15 | 3.82 |
| Completeness [%] | 97.2 | 95.4 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295.15 | 0.2M Zinc Acetate, 0.1M MES, 15% v/v Ethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
