3SJD
Crystal structure of S. cerevisiae Get3 with bound ADP-Mg2+ in complex with Get2 cytosolic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.640, 209.750, 133.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.230 - 4.600 |
| R-factor | 0.326 |
| Rwork | 0.323 |
| R-free | 0.38800 |
| Structure solution method | PHASER |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.005 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.600 | 4.850 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Number of reflections | 11890 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 40% (v/v) 1,2-propanediol, 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
