3SJB

Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Wavelength(s)	0.9395
Spacegroup name	P 65 2 2
Unit cell lengths	112.900, 112.900, 310.481
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.887 - 3.300
R-factor	0.197
Rwork	0.195
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2woj
RMSD bond length	0.009
RMSD bond angle	1.194
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.300	3.480
High resolution limit [Å]	3.300	3.300
Number of reflections	18481
Completeness [%]	99.7	95.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1 M LiCl, 20% PEG 6000, 0.1 M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K