3SJA
Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Collection date | 2004-11-27 |
| Wavelength(s) | 0.9395 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 204.120, 91.460, 149.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.300 - 3.000 |
| R-factor | 0.1834 |
| Rwork | 0.181 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.157 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 56397 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 1 M LiCl, 20% PEG 6000, 0.1 M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
