3SJ2
A Crystal Structure of a Model of the Repeating r(CGG) Transcript Found in Fragile X Syndrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.660, 32.320, 69.442 |
| Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
| Resolution | 27.543 - 1.360 |
| R-factor | 0.1571 |
| Rwork | 0.154 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.783 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.560 | 27.560 | 1.460 |
| High resolution limit [Å] | 1.360 | 4.120 | 1.360 |
| Number of reflections | 24111 | ||
| Completeness [%] | 98.6 | 93.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Potassium Chloride, 0.01 M Magnesium Chloride, 0.05 M Tris.HCl, 30%(v/v)PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
