3SIY
Crystal structure of a DUF1989 family protein (TM1040_0329) from SILICIBACTER SP. TM1040 at 1.35 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97971,0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.907, 48.132, 144.732 |
| Unit cell angles | 90.00, 98.44, 90.00 |
Refinement procedure
| Resolution | 58.798 - 1.350 |
| R-factor | 0.1387 |
| Rwork | 0.137 |
| R-free | 0.17150 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.798 | 58.798 | 1.420 |
| High resolution limit [Å] | 1.350 | 4.270 | 1.350 |
| Rmerge | 0.086 | 0.042 | 0.625 |
| Total number of observations | 48027 | 146252 | |
| Number of reflections | 152479 | ||
| <I/σ(I)> | 11.1 | 12.7 | 2.3 |
| Completeness [%] | 85.8 | 98.5 | 100 |
| Redundancy | 6.6 | 8.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.0% PEG 3350, 0.1M Magnesium Chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
