3SI6
RB69 DNA Polymerase Triple Mutant (L561A/S565G/Y567A) Ternary Complex with dUpNpp and a Deoxy-terminated Primer in the presence of Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.458, 120.195, 131.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.314 - 1.850 |
| R-factor | 0.18451 |
| Rwork | 0.183 |
| R-free | 0.21685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.314 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.124 | 0.827 |
| Number of reflections | 100690 | |
| <I/σ(I)> | 10.1 | 1.25 |
| Completeness [%] | 98.6 | 99 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH VAPOR DIFFUSION | 6.5 | 293.15 | 150 mM calcium chloride, 1% w/v PEG350 MME, 100 mM sodium cacodylate, pH 6.5, MICROBATCH VAPOR DIFFUSION, temperature 293.15K |
