3SHX
Frog M-ferritin with magnesium, L134P mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.775, 183.775, 183.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.380 - 1.350 |
R-factor | 0.13881 |
Rwork | 0.137 |
R-free | 0.16299 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.030 |
RMSD bond angle | 2.468 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.071 | 0.807 |
Number of reflections | 59574 | |
<I/σ(I)> | 19.5 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.1 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2M MGCL2, 0.1M BICINE, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |