3SHS
Three N-terminal domains of the bacteriophage RB49 Highly Immunogenic Outer Capsid protein (Hoc)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.540, 36.731, 62.693 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 27.691 - 1.951 |
R-factor | 0.2231 |
Rwork | 0.218 |
R-free | 0.26730 |
Structure solution method | SIRAS, 3-wavelength data from a Pt derivative |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.700 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 18095 | |
<I/σ(I)> | 23.14 | 4.1 |
Completeness [%] | 50.9 | 15 |
Redundancy | 6.2 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 800, 200 mM MgCl, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 800, 200 mM MgCl, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |