3SHM
Structure-function Analysis of Receptor Binding in Adeno-Associated Virus Serotype 6 (AAV-6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 |
| Unit cell lengths | 258.357, 258.357, 612.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.672 - 3.019 |
| R-factor | 0.2732 |
| Rwork | 0.273 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AAV2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.451 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.672 | 40.000 | 3.430 |
| High resolution limit [Å] | 3.019 | 4.330 | 3.019 |
| Rmerge | 0.080 | 0.072 | 0.275 |
| Number of reflections | 68828 | ||
| <I/σ(I)> | 4.4 | 6.77 | 1.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.3 | 298 | 4-6% PEG6000, 100 mM HEPES, 50 mM magnesium, pH 7.3, HANGING DROP, temperature 298K |
