3SHB
Crystal Structure of PHD Domain of UHRF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-03-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.2823 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 36.609, 36.609, 219.765 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.704 - 1.800 |
R-factor | 0.2107 |
Rwork | 0.210 |
R-free | 0.22780 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 8535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M TRIS hydrochloride (pH8.5), 30% w/v Polyethylene glycerol 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |