3SHB

Crystal Structure of PHD Domain of UHRF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.2823
Spacegroup name	P 61 2 2
Unit cell lengths	36.609, 36.609, 219.765
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.704 - 1.800
R-factor	0.2107
Rwork	0.210
R-free	0.22780
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.322
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	8535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M TRIS hydrochloride (pH8.5), 30% w/v Polyethylene glycerol 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K