3SH4
Laminin G like domain 3 from human perlecan
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.950, 54.655, 64.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 1.500 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21290 |
| Structure solution method | MAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.234 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.670 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 22563 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 3.5M sodium formate, 0.1 sodium acetate pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
