3SH3
Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.47 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.820, 66.873, 107.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.431 - 2.300 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.046 |
| Number of reflections | 9238 |
| Completeness [%] | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.01 M Nickel (II), 0.1 M Tris pH 8.0, 1.0 M lithium sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
