3SGC
Crystal Structure of Apo Aminoglycoside-2''-Phosphotransferase Type IVa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-03-04 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.892, 61.725, 102.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.750 - 2.050 |
R-factor | 0.22165 |
Rwork | 0.219 |
R-free | 0.26207 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 1.890 | 2.050 |
Number of reflections | 21272 | |
<I/σ(I)> | 4.1 | |
Completeness [%] | 94.0 | 97.2 |
Redundancy | 10.9 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 50 mM Tris-HCl, 200 mM NaCl, 15% PEG2000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |