3SGC
Crystal Structure of Apo Aminoglycoside-2''-Phosphotransferase Type IVa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.892, 61.725, 102.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.750 - 2.050 |
| R-factor | 0.22165 |
| Rwork | 0.219 |
| R-free | 0.26207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.890 | 2.050 |
| Number of reflections | 21272 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 94.0 | 97.2 |
| Redundancy | 10.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 50 mM Tris-HCl, 200 mM NaCl, 15% PEG2000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
