3SGA

STRUCTURES OF PRODUCT AND INHIBITOR COMPLEXES OF STREPTOMYCES GRISEUS PROTEASE A AT 1.8 ANGSTROMS RESOLUTION. A MODEL FOR SERINE PROTEASE CATALYSIS

Experimental procedure

Spacegroup name	P 42
Unit cell lengths	55.180, 55.180, 54.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.12
RMSD bond length	0.017 *
RMSD bond angle	0.036 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.034 *
Total number of observations	16005 *
Number of reflections	15228 *
Completeness [%]	71.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	equilibrium dialysis *	4.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%(w/v))
2	1	drop		1.3 (M)