3SG6
Crystal Structure of Dimeric GCaMP2-LIA(linker 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-02 |
Detector | RIGAKU SATURN 92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.976, 47.491, 68.673 |
Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
Resolution | 27.740 - 1.700 |
R-factor | 0.20573 |
Rwork | 0.203 |
R-free | 0.24942 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ek7 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.018 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 40438 | |
<I/σ(I)> | 15.9 | 1.6 |
Completeness [%] | 93.9 | 73.9 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |