3SG0
The crystal structure of an extracellular ligand-binding receptor from Rhodopseudomonas palustris HaA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.494, 70.301, 98.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.625 - 1.201 |
| R-factor | 0.1502 |
| Rwork | 0.150 |
| R-free | 0.16160 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.096 | 0.557 |
| Number of reflections | 134515 | |
| <I/σ(I)> | 44.6 | 2.5 |
| Completeness [%] | 99.7 | 95.9 |
| Redundancy | 7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.6M Sodium Citrate Tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
