3SFZ
Crystal structure of full-length murine Apaf-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.650, 113.070, 243.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.2355 |
Rwork | 0.235 |
R-free | 0.29800 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.100 | |
High resolution limit [Å] | 3.000 | 20.000 | 3.000 |
Rmerge | 0.085 | 0.043 | 0.405 |
Number of reflections | 40531 | 143 | 3711 |
<I/σ(I)> | 14.26 | 21.61 | 3.53 |
Completeness [%] | 98.6 | 80.8 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 1.4 M sodium malonate, 4% v/v gamma-butyrolactone, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |