3SEO
Crystal structure of VopL C terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2010-06-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.180, 89.851, 103.007 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.153 - 2.305 |
R-factor | 0.2254 |
Rwork | 0.221 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.041 | 0.026 | 0.785 |
Number of reflections | 23092 | ||
<I/σ(I)> | 18.3 | ||
Completeness [%] | 99.1 | 85.2 | 99.7 |
Redundancy | 5.3 | 4.6 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG1500, 100mM Tris pH=7.5, 100mM NaCl, 2mM TCEP, vapor diffusion, hanging drop, temperature 293K |